delly2-lr-germline

Identify germline variants in long read DNA sequencing BAM files using Delly2

usage

nexus run --nf-workflow variant_calling_delly2-lr-germline.nf \
    -c nextflow.config \
    -w work/ \
    --samples_tsv_file samples.tsv \
    --output_dir results/ \
    --reference_genome_fasta_file /path/to/file.fasta \
    --delly2_exclude_tsv_file /path/to/file.tsv \
    --params_delly2lr "--mapqual 20 --technology pb"

Note

Nextflow config files are available here. Use the config file that matches your installed nexus version (e.g. nexus_v0.2.0_nextflow_slurm.config).

parameters

parameter	description
--samples_tsv_file	TSV file with the following columns: ‘sample_id’, ‘bam_file’, ‘bam_bai_file’.
--output_dir	Directory to which output files will be copied.
--reference_genome_fasta_file	Reference genome FASTA file.
--exclude_tsv_file	Delly2 ‘call’ –exclude TSV file.
--params_delly2lr	Delly2 ‘lr’ parameters (default: ‘“–mapqual 20 –technology pb”’). Note that the parameters need to be wrapped in quotes.