circexplorer2

Identify circular RNAs using CIRCexplorer2

usage

nexus run --nf-workflow variant_calling_circexplorer2.nf \
    -c nextflow.config \
    -w work/ \
    --samples_tsv_file samples.tsv \
    --output_dir results/ \
    --reference_genome_fasta_file /path/to/file.fasta \
    --circexplorer2_gene_annotation_txt_file /path/to/file.txt \
    --params_circexplorer2_parse "-t STAR" \
    --params_circexplorer2_annotate ""

Note

Nextflow config files are available here. Use the config file that matches your installed nexus version (e.g. nexus_v0.2.0_nextflow_slurm.config).

parameters

parameter	description
--samples_tsv_file	TSV file with the following columns: ‘sample_id’, ‘input_file’.
--output_dir	Directory to which output files will be copied.
--reference_genome_fasta_file	Reference genome FASTA file.
--circexplorer2_gene_annotation_txt_file	CIRCexplorer2 gene annotation TXT file.
--params_circexplorer2_parse	CIRCexplorer2 parse parameters (default: ‘“-t STAR”’). Note that the parameters need to be wrapped in quotes.
--params_circexplorer2_annotate	CIRCexplorer2 annotate parameters (default: ‘““’). Note that the parameters need to be wrapped in quotes.