isoquant

Characterize isoforms using Isoquant

usage

nexus run --nf-workflow isoform_characterization_isoquant.nf \
    -c nextflow.config \
    -w work/ \
    --samples_tsv_file samples.tsv \
    --output_dir results/ \
    --reference_genome_fasta_file /path/to/file.fasta \
    --reference_genes_gtf_file /path/to/file.gtf \
    --params_isoquant "--data_type pacbio_ccs --sqanti_output --high_memory --complete_genedb"

Note

Nextflow config files are available here. Use the config file that matches your installed nexus version (e.g. nexus_v0.2.0_nextflow_slurm.config).

parameters

parameter	description
--samples_tsv_file	TSV file with the following columns: ‘sample_id’, ‘fastq_file’.
--output_dir	Directory to which output files will be copied.
--reference_genome_fasta_file	Reference genome FASTA file.
--reference_genes_gtf_file	Reference genes GTF file.
--params_isoquant	isoquant parameters (default: ‘“–data_type pacbio_ccs –sqanti_output –high_memory –complete_genedb”’). Note that the parameters need to be wrapped in quotes.